Creating TRPV1 knock down by lentiviral transgenesis

Multiple gene knock-down by a single lentiviral vector expressing an array of short hairpin RNAs

Efficient transgenesis in farm animals by lentiviral vectors.

Microinjection of DNA is now the most widespread method for generating transgenic animals, but transgenesis rates achieved this way in higher mammals are extremely low. To address this longstanding problem, we used lentiviral vectors carrying a ubiquitously active promoter (phosphoglycerate kinase, LV-PGK) to deliver transgenes to porcine embryos. Of the 46 piglets born, 32 (70%) carried the tr...

Vpx-Independent Lentiviral Transduction and shRNA-Mediated Protein Knock-Down in Monocyte-Derived Dendritic Cells

The function of dendritic cells (DCs) in the immune system is based on their ability to sense and present foreign antigens. Powerful tools to research DC function and to apply in cell-based immunotherapy are either silencing or overexpression of genes achieved by lentiviral transduction. To date, efficient lentiviral transduction of DCs or their monocyte derived counterparts (MDDCs) required hi...

07-P022 Applications of lentiviral transgenesis in the chick

We have previously shown that lentiviral vectors can be used to generate transgenic chickens efficiently and that expression of introduced transgenes is not silenced on transmission through the germline. Transgene expression can be targeted in the predicted tissue-restricted manner, for example: muscle-specific expression is restricted to skeletal muscle using the rat myosin light chain 3 gene ...

Lentiviral vectors: are they the future of animal transgenesis?

Lentiviral vectors have become a promising new tool for the establishment of transgenic animals and the manipulation of the mammalian genome. While conventional microinjection-based methods for transgenesis have been successful in generating small and large transgenic animals, their relatively low transgenic efficiency has opened the door for alternative approaches, including lentiviral vectors...